РУАЭСТ (RUAEST)
APPLICATION OF FOSTER – NUEMANN METHOD FOR ESTIMATION OF EFFICIENCY OF PROTEIN ENZYMATIC HYDROLYSIS OF BLACK SEA ATHERINA
The estimation of efficiency of technological modes of protein enzymatic hydrolysis of the black sea atherina by Foster – Nuemann method is made. By the linearization of experimental curves the apparent Michaelis’s constant for substrates with different moisture based on the black sea atherina muscle tissue was obtained. From the experimental Michaelis’s constant values comparison, it follows that in a system with high humidity Protosubtilin G3x enzymes affinity to substrate proteins is higher than in natural humidity systems. The obtained value of Michaelis constant for the theoretical process allows to assert that the enzymatic hydrolysis of the considered enzyme-substrate systems with the addition of 50 % of water does not meet any damping