One of effective methods for estimation of inbred lines of winter rye is the diallel analysis allowing to spread out genotypic variance to general (GCA) and specific (SCA) combinational abilities and to estimate quantitatively the contribution of the basic gene interactions in expression of traits. The purpose of our research was studying genetic features of inbred lines of rye by means of diallel crossings. Tested 5 inbred lines of winter rye and 10 interline F1 hybrids were obtained according to incomplete diallel scheme (B. Griffing, 1956).