THE STUDY OF FACTORS AFFECTED THE GENE TRANSFER EFFICIENCY IN CHICKEN EMBRYONIC CELLS BY APPLICATION OF LENTIVIRAL VECTORS

Lentivirus-mediated gene transfer is being the one of the attractive method for genetic modification of chicken (S.C. Chapman et al., 2005; C.A. Smith et al., 2009; N.A. Volkova et al., 2013). However, the efficiency of thansgenesis of the chicken embryonic cells has been shown to be relatively low. Therefore, a large number (60,000 to 100,000) of embryonic cells at the start of incubation and the virus preparations with high titers (about 10particles per milliliter) remain one of a crucial problem the researchers are facing with when try to achieve a satisfactory transgene introduction. The aim of the present study was to determine the optimal conditions for production and application of the modified lentiviral vector system of second generation for the transgenesis of chicken embryos.

• ZINOVIEVA N.A.
• Volkova N.A.
• Fomin I.K.
• Mennibaeva E.R.
• Tomgorova E.K.
• Vetokh A.N.

• Искусство. Декоративно-прикладное искусство. Фотография. Музыка. Игры. Спорт

• Естественные науки
• Сельское хозяйство. Лесоводство