The characteristic of the allele pool of the Large White and Yorkshire pig breeds using 11 microsatellites (S0155, S0355, S0386, SW72, SW951, S0101, SW240, SW857, S0228, SW911 and SW936) was done. The study was performed on 17 pig populations of Russian, Danish, Hungarian, Canadian, Lithuanian, German, Ukrainian and French origins (n = 837). The data were analyzed using GenAlEx (v. 6.4), Structure (v. 2.3.1) and Past (v. 2.15) software. It was shown that 24.2 per cent of the total microsatellite diversity was due to intra population differences (Rst = 0.242, p = 0.01). Based on Fst and Rst (AMOVA) values the greater contribution of geographically distant and local populations into microsatellites diversity was observed. The cluster analysis performed according J.K. Pritchard (2000) and M. Nei (1983) showed that the cluster structure was in concordance with populations' origin.