Environmentally friendly products are one of the most important tasks in agriculture. Microorganisms of different origin are used for the production of biological preparations pathogenic to pests. The preparations based on entomopathogenic bacteria Bacillus thuringiensis (Bt) are of the most interest due to their selective action, safety for humans, warm-blooded animals, beneficial insects and the environment. The departmental collection of beneficial microorganisms for agricultural purposes (RCAM collection, St. Petersburg) stores B. thuringiensis with known cultural, morphological, physiological, biochemical, technological, insecticidal and other properties.